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PZ - Enzymes, Exoglycosidases

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Sialidase V Glyko® Sialidase V™ Releases alpha(2-3,6,8)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Source: Native from Vibrio cholerae. Specificity: The enzyme is specific for N- acetyl (Neu5Ac) or N-glycolyl (Neu5Gc) neuraminic acid at the non-reducing terminus in a2-3,6 or 8 linkages to various monosaccharides (e.g. galactose, neuraminic acid (NeuAc-NeuAc), N-acetyl-glucosamine, N-acetyl-galactosamine). These are found in a wide range of oligosaccharides, polysaccharides and glycoconjugates such as glycoproteins and glycolipids. The rate of hydrolysis of a2-3 over a2-6 and/or a2-8 linkages is approximately 3 fold.
CAT NO: PZGKX-5020 QTY: 1 U
Beta (1-4,6)-Galactosidase (jack bean) Glyko® Beta(1-4,6)-Galactosidase. Hydrolyses non-reducing terminal Gal-beta(1-6)GlcNAc and Gal-beta(1-4)GlcNAc. Gal beta(1-3)GlcNAc is hydrolyzed very slowly. The relative rates of hydrolysis are: Beta (1-6), 100%; Beta (1-4) 75%; and Beta (1-3), 1%. Gal-Beta (1-6) and Gal-Bata (1-3) can also be released from other aglycones although the rates of hydrolysis are variable.
CAT NO: PZGKX-5012 QTY: 5 U
Beta (1-3,4)-Galactosidase (bovine testis) Glyko® Beta(1-3,4)-Galactosidase. Hydrolyzes non-reducing terminal galactose beta(1-3) and beta(1-4) linkages. Can be used in conjunction with otherBeta-galactosidases for exoglycosidase sequencing.
CAT NO: PZGKX-5013 QTY: 0. 5 U
Beta (1-4)-Galactosidase (Streptococcus pneumoniae) Glyko® Beta(1-4)-Galactosidase. The enzyme releases non-reducing terminal beta(1-4)-linked galactose from oligosaccharides and glycoproteins. This specificity is only evident at enzyme concentrations < 100mU/ml. At higher concentrations, hydrolysis of Beta (1-3)-linked galactose occurs.
CAT NO: PZGKX-5014 QTY: 200 mU
Alpha (1-2)-Mannosidase (Aspergillus satoi) Glyko® Alpha(1-2)-Mannosidasse. Releases alpha (1-2)-linked mannose residues from the non-reducing terminus of oligosaccharides. Used in exoglycosidase sequencing. 2 mU is equal to 50µU as previously reported by Glyko, Inc. Source: Aspergillus saitoi. Specificity: The enzyme specifically cleaves Alpha (1-2)-linked mannose from the non-reducing terminus of glycans. As such it is extremely valuable in identifying this type of linkage and, in particular, the presence of high mannose-type N-glycans which are digested to the Man-5 structure.
CAT NO: PZGKX-5009 QTY: 2 mU
Alpha (1-2,3,6)-Mannosidase (jack bean) Glyko® Alpha(1-2,3,6)-Mannosidase (jack bean). Releases non-reducing terminal alpha (1-2,3,6)-linked mannose from oligosaccharides. The enzyme exhibits some kinetic preference for Alpha (1-2,3)-linked mannose over Alpha(1-6)-linked mannose.
CAT NO: PZGKX-5010 QTY: 10 U
Beta-Mannosidase (Helix pomatia) Glyko® Beta-Mannosidase. Releases non-reducing terminal beta(1-4)-linked mannose from oligosaccharides. The enzyme hydrolyzes non-reducing terminal Beta (1-4)- linked mannose residues with relative specificity; Beta 1-3 and Beta 1-6 linked mannose residues are reported to be hydrolyzed at much lower rates of 0.04 and 0.1 % respectively.
CAT NO: PZGKX-5015 QTY: 1 U
Beta N-Acetylhexosaminidase Glyko® Beta N-Acetylhexosaminidase. Releases non-reducing terminal Beta(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Source: Jack bean.
CAT NO: PZGKX-5003 QTY: 5 U
Alpha (1-2,3,4,6)Fucosidase (bovine kidney) Glyko® Alpha(1-2,3,4,6) Fucosidase (bovine kidney). Broad specificity; releases non reducing terminal alpha(1-6) core linked fucose more efficiently than other alpha fucose linkages.
CAT NO: PZGKX-5006 QTY: 500 mU
Alpha-(1-3,4,6)-Galactosidase from Green coffee bean. Glyko® Alpha(1-3,4,6)-Galactosidase releases non-reducing terminal alpha(1-3,4,6)-linked galactose from oligosaccharides. NOTE: Highly purified; beta-galactosidase contaminating activity not detectable (extended incubation with 2-AB NA2). Specificity: Coffee bean a-galactosidase cleaves non-reducing terminal galactose residues linked a1-3, a1-4 and a1-6 to polysaccharides, glycoproteins and glycans. The precise specificity of the enzyme is somewhat dependent on the nature of the glycoconjugate.
CAT NO: PZGKX-5007 QTY: 5 U
N-Glycanase PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. "A & B" pack sizes ship with WS0010 5x N-Glycanase Reaction Buffer. The D pack size does not include the buffers. If buffers are required please request them.
CAT NO: PZGKE-5006D QTY: 1000 mU
Beta-(1-2,3,4,6) Hexosaminidase Sequencing (Jack Bean) Glyko® Beta(1-2,3,4,6) Hexosaminidase, Sequencing-grade. Releases non-reducing terminal beta(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Supplied at a higher concentration than GKX-5003. Higher enzyme concentrations may be necessary to remove bisecting ß(1-4)-linked GlcNAc residues in complex N-glycans. Source: Jack bean.
CAT NO: PZGKX-5023 QTY: 15 U
Sialidase C Glyko® Sialidase C™ (recombinant from Clostridium perfringens, expressed in E. coli)
CAT NO: PZGK80030 QTY: 1 U
Sialidase A Glyko® Sialidase A™ Glyko® Sialidase A (recombinant gene from Arthrobacter ureafaciens, expressed in E. coli)
CAT NO: PZGK80040 QTY: 1 U
Sialidase A Sampler Kit Glyko® Sialidase A™ Sampler KitThese products are available for purchase separately in 1-unit packs sizes under individual product codes.
CAT NO: PZGK80043 QTY: 1 ea
Sialidase A-51 Glyko® Sialidase A™ 51 Lower MW version of GK80040 for challenging digestions. Releases alpha(2-3)-alpha(2-6)- alpha(2-8), and alpha (2-9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. Also capable of releasing N-glycolylneuraminic acid. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue. If you think a lower MW sialidase will help desialylate your glycans, you can also try our 88, 66 and 51 kDa Sialidase A Sampler Kit.
CAT NO: PZGK80045 QTY: 1 U
Sialidase A-66 Glyko® Sialidase A™ 66. Lower MW version of GK80040 for challenging digestions.
CAT NO: PZGK80046 QTY: 1 U
Beta N-Acetylhexosaminidase/HEXase I Glyko® Beta-N-Acetylhexosaminidase/HEXase I (recomb from Streptococcus pneumoniae, expressed in E. coli)
CAT NO: PZGK80050 QTY: 1.6 U
Sialidase S. Glyko® Sialidase S™ Recombinant from Streptococcus pneumoniae, expressed in E. coli. 6xHis-tagged.
CAT NO: PZGK80021 QTY: 3 U
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