BCIP/NBT plus (5-bromo-4-chloro-3-indolyl phosphate [BCIP 0.692 mmol/L]/nitroblue tetrazolium (NBT 0.734 mmol/L)
One component. Stable for 36 months.
This reagent may be used for both immunoblotting and immunohistochemical procedures. The system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Moss' single component substrate is more sensitive than existing products and produces little or no background staining.