3’-O-Acetyl-2’,7’-bis(carboxyethyl)-4 or 5-carboxyfluorescein, diacetoxymethyl ester. CAS:117464-70-7
BCECF is the most widely used intracellular pH probe. Dr. Tsien and others improved this carboxyfluorescein by introducing two extra carboxylates that allow it to be retained better by the cell. BCECF is highly water-soluble because it has 4 to 5 negative charges at neutral pH; it becomes difficult to pass through the cell membrane after loading.