PNGase F (native)

Glyko® PNGase F (native), Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-ß-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Also available as a recombinant product, see GKE-5006A.


Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-ß-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Also available as a recombinant product, see GKE-5006A.
Native, purified from Elizabethkingia meningosepticum. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum [1].

Specificity:
PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core a1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.

PNGase F (native)
PZGKE-5003
1 U