Sensitive, rapid, automatable assay for quantitation of total sialic acid, either free or released from intact glycoproteins; suitable for product release or process analytical technology (PAT). Single-well procedure for fast and simple processing Broad range of detection, 40 pmol - 1,000 pmol (fluorescence) and 1 nmol - 5 nmol (absorbance) Enzymatic cleavage allows rapid analysis (~75 minutes) with minimal, if any, degradation of sialic acid 4 different kit options to meet your specific needs GS300 - High troughput Automatable, 96-well microplate format with fluorescence detection Sufficient for ~90 data points GF57 - Research Introduction to the method with a known glycoprotein and confirmation of enzymatic vs. acid-catalyzed release Three assay formats: 96-well microplate format using absorbance or fluorescence, and test tubes/cuvettes with a standard fluorometer 2 sets of 30 data points GS300LA - Low enzyme Cost-effective version for easily desialylated proteins GS300WD - Routine measurements Minimal sialidase with extended digestion times (up to 3 hours) for cost-effective analysis Single-use kit (30 data points) for small numbers of samples
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