Jun 04th, 2015
Development of an Instant Glycan Labeling Dye for High Throughput Analysis by Mass Spectrometry
Glycosylation of biotherapeutic proteins is frequently a critical quality attribute; therefore the characterization of glycans on biotherapeutics is an important activity in the development process. A common approach is to release and label N-glycans
with a tag to allow for fluorescence detection; a process that often requires numerous hours to complete. Unfortunately,
many of the most commonly used fluorescent tags have poor MS sensitivity.
- We present a novel instant glycan labeling reagent for MS
(IMS) that provides markedly increased MS and FLR sensitivity
- The workflow utilizes a rapid in-solution digestion, instant labeling, and cleanup of excess IMS label
- IMS is shown to lend itself to both rapid (5-minute) and high-resolution (60-minute) HILIC methods for N-glycan separation, allowing flexibility for screening applications and in-depth characterization
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