Development of an Instant Glycan Labeling Dye for High Throughput Analysis by Mass Spectrometry

Glycosylation of biotherapeutic proteins is frequently a critical quality attribute; therefore the characterization of glycans on biotherapeutics is an important activity in the development process. A common approach is to release and label N-glycans with a tag to allow for fluorescence detection; a process that often requires numerous hours to complete. Unfortunately, many of the most commonly used fluorescent tags have poor MS sensitivity.

• We present a novel instant glycan labeling reagent for MS (IMS) that provides markedly increased MS and FLR sensitivity
• The workflow utilizes a rapid in-solution digestion, instant labeling, and cleanup of excess IMS label
• IMS is shown to lend itself to both rapid (5-minute) and high-resolution (60-minute) HILIC methods for N-glycan separation, allowing flexibility for screening applications and in-depth characterization



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