The Gly-X™ N-Glycan Rapid Release and Labeling with InstantPC™ kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. After a simple clean-up step, the glycan samples are ready for analysis by UHPLC, LC-MS, MS/MS and other methods. With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows.
Other benefits include:
- Small molecule size for improved chromatographic peak resolution
- Flexible, high-throughput format: process 1 to 96 samples
- Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Contains:
- PZGX96-100 Gly-X Deglycosylation Module
- PZGX96-101 Gly-X InstantPC Labeling Module
- PZGX96-102 Gly-X InstantPC Clean-up Module
Equipment and reagents provided by user:
- 96-well Thermocyler or two independent heat blocks, set for 90 °C and 50 °C
- Thermocylcer (Corning THERM-1001, 110V; THERM-1000, 230V)
- Note: Two GlykoPrep Heaters, WS0271, can be fitted with VWR 13259-260 Modular Heating Blocks
- Vacuum manifold (Millipore MSVMHTS00-HTS)
- Vacuum pump (Millipore WP6211560, 110 V; WP6122050, 220V)
- Formic acid, MS-grade (Fisher A117-50 recommended)
- Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
PRODUCTS
PZ - Gly-X With Instant PC
